中藥之化學鑑定研究-葛根與黃芩之基原辨別（3-3）

許順吉
國立台灣師
白芍屬補血藥，有補血、緩急止痛功能；赤芍屬活血祛瘀藥，具清熱、涼血、活血祛瘀功效。醫藥書籍記載，桂枝茯苓丸應該用赤芍、芍藥甘草湯應該用白芍，可知使用正確原料藥材，是對症下藥的先決條件。本研究用Cosmosil 5C18-MS管柱，以磷酸鹽溶液配合氰甲烷、甲醇等有機溶劑為沖提液，進行分析，結果顯示albiflorine與paeoniflorine的比值是兩者的分辨點，赤芍幾近於0，白芍可以明顯看到albiflorine的存在，此法最重要的是可應用於藥材、單方與複方。CE也可用為檢測工具。本研究篩驗含白芍製劑14方，含赤芍製劑3方。
日本漢方限用日連，中華藥典則用川連、雅連，近來日本厚生省開始檢討兩者應用於製劑有無差異。我們用ODS-80TM管柱，醋酸鹽與氰甲烷沖提液分析，可以明確看出兩者的finger-print差異。日連不含epiberberine，川連則有berberine的七分之一量，CE更適合於該等生物鹼的判別。本方法應用於藥材、單方、複方製劑的基原辨識，皆無困難。本研究篩驗含黃連製劑10方。
古籍記述吳茱萸藥材「開口者良、閉口者悶」，長期服用閉口者，可能引起胸悶的副作用。本研究以Cosmosil 5C18-MS管柱，醋酸鹽、氰甲烷及甲醇沖提液分離，用其藥理活性成分evodiamine及dehydroevodiamine分辨之，開口者evodiamine較多，閉口者反之，可實際應用於市售品的檢測。本研究篩驗市售吳茱萸湯濃縮製劑。
藥材主要組成成分的安定性亦經測定，一般而言，都隨時間增加而遞減，但看不出有明顯的規則性。本研究針對吳茱萸及芍藥藥材作系列檢討。
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『Study on the identification of Chinese herbal drugs by chemical analysis』

Shuenn-Jyi Sheu
Department of Chemistry, National Taiwan Normal University
White peony is a hemopoietic drug capable of supplementing blood, alleviating urgency and arresting pain, while red peony belongs to the category of blood enlivening drugs capable of clearing heat, cooling blood, enlivening blood and removing stagnant blood. From the medical literature, the herbal formula Cinnamon and Hoelen Combination should contain red peony as component, and the formula Peony and Licorice Combination should contain white peony. Thus, the use of correct drug materials is a prerequisite in the practice of “prescribe according to symptom complex”. This study employed Cosmosil 5 C18-MS column and used phosphate salt solution together with acetonitrile and methanol as eluent in the analysis of the herbs. The result shows that the distinction between the two herbs lies in the albiflorine to paeoniflorine ratio, which is almost zero in red peony; whereas albiflorine is found to exist markedly in white peony. The most important with this method is its application to the
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