連續十二週經口投與歸脾湯對老年大鼠的影響

林文川
私立中國醫
中醫傳統方劑歸脾湯主要用於健忘、失眠及貧血等。最進的研究指出，對年輕鼷鼠歸脾湯有加強學習及記憶的能力和抗氧化的作用。基於這些實驗結果，本研究計畫同時進行歸脾湯亞急性毒性及抗衰老的實驗，使用15月齡的大鼠，經口連續投與歸脾湯12週。在實驗期間進行下列觀察及測定：死亡率及一般症狀、體重、飲食及飲水量、血壓及心跳。實驗終了進行血液學及病理學檢驗、血清生化學檢驗及尿液分析。另外也測定衰老指標如：腦部及心臟脂褐素含量、尾腱及皮膚膠原蛋白含量、各臟器脂質過氧化程度。也測定血液、腦部、肝臟中酵素類及非酵素類抗氧化劑的含量。測定B、T淋巴細胞及自然殺手細胞於實驗前及實驗終了的數目。
關鍵字：歸脾湯、亞急性毒性、抗衰老、抗氧化

Effects of 12-weeks repeated oral administration of the Gui-pi-tang on aged rats

Wen-Chuan Lin
China Medical College

Gui-pi-tang (GPT) , a traditional Chinese medicine, had been used to treat amnesia, insomnia and aplastic anemia. Recently, it has been shown that GPT exhibited antioxidant action and improvement of learing and memory in young mice. According to these results, we design an experiment to study the subacute toxicity and anti-aging effect of GPT. This study is performed by the administration of a GPT for 12 weeks in aged rats (15 months). The following measurements and examinations are performed on all animals during the study period: mortality and general condition, body weight, food and water consumption, blood pressure and heart rate. The hematological and pathological examinations, blood chemistry tests and urinalysis are performed at the end of treatment. In addition, the parameters of aging , such as lipofuscin content in the brain and heart, collagen content in the tail and skin, lipid peroxidation content in the various organs, are also determined at the end of. The enzyme
關鍵字：Gui-pi-tang, Subacute toxicity, Anti-aging, Antioxidant