癲癇的中西醫學整合研究--中藥方柴胡龍骨牡蠣湯抗癲癇效果及機轉的電生理研究 (5-2)

蔡景仁
成功大學醫
傷寒論的柴胡加龍骨牡蠣湯是最具潛力的抗癲癇經方，據此加入天麻、鉤籐兩味中藥而成“加減柴胡龍骨牡蠣湯”。本研究依美國國家研究開發新型抗癲癇藥物實施準則，從事此中藥方的篩選研發工作。第一年進行此方抗癲癇性質的鑑定、量化及鑑別研究。第二年，我們將從事此方的抗癲癇電氣生理效果及機轉研究。實驗動物採雄性Sprague-Dawley鼠，進行對神經元電壓閥門(voltage-gated)及神經傳遞物質的離子閥門(neurotransmitter-gated)的研究。實驗將分1)腦細胞外2)腦細胞內兩部份的神經生理基礎分析，來評估“加減柴胡龍骨牡蠣湯”對腦細胞膜電位及離子電流改變的效應。

資料的收集和分析則透過pClamp 6.0 (Axon Instruments)將數據輸入電腦PC486內進行，所有資料以平均值±標準誤差表示，統計分析以Students t-test來檢測並定。 

關鍵字：癲癇、柴胡龍骨牡蠣湯、神經電生理

The Integration of Chinese and Western Medicine in Epilepsy Research The Electrophysiological Study of Antiepileptic Effect and Mechanism of Action of the Chinese Herb-Chai-Hu-Long-Ku-Mu-Li-Tan

Tsai Jing-Jane
Epilepsy research center, Cheng Kung University Hospital
The decoction of Chai-Hu-Long-Ku-Mu-Li-Tan from the description in the Chinese medical book “Shang Han Lun” has the potential antiepileptic effect. Gouteng and Tian-Ma are two Chinese herbal drugs that have also been used as an antiepileptic drugs for a long time. We modified the components of Chai-Hu-Long-Ku-Mu-Li-Tan and combined them with Gouteng and Tian-Ma to formulate a new mixture. According to the guidelines of the Anticonvulsant Screening Projet (ASP) in U.S.A, we conduct the tests of the antiepileptic properties of the new mixture of modified “Chai-Hu-Long-Ku-Mu-Li-Tan”. In the first year, the testing procedures of identification, quantification and differentiation studies are used extensively for evaluating the antiepileptic properties of this mixture. In this project, we adopt the animal model of using the brain slice of male Sprague-Dawley rats to test the antiepileptic effects of this mixture with an aid of electrophysiological studies, including the evaluation of it
關鍵字：Epilepsy, Chai-Hu-Long-Ku-Mu-Li-Tan, Neuroelectrophysiology