茵陳蒿湯之抗氧化作用的研究

陳明豐 
台南巿立醫
最近游離基與過氧化脂質被發現與各種化學藥物引起的肝傷害有關，並且是病毒性肝炎、脂肪肝、缺血或休克後的肝傷害之誘發或增惡因子。因此，能消除游離基與過氧化脂質之抗氧化劑被期待為治療肝疾患的藥物。另一方面，很多治療肝病的單味中藥被發現其作? 徆鷘鄍D要與抗氧化活性有密切的關係。我們在過去的研究中發現，十二種常用保肝中藥方劑中以茵陳蒿湯的抗氧化作用最為明顯。由於茵陳蒿湯經常被使用於急性肝炎合併黃疸病人，因此，我們擬進一步探討茵陳蒿湯對急性肝炎合併黃疸病患之全血超氧陰離子(superoxide anion)及過氧? B（H2O2）等氧自由基的消除作用。全血超氧陰離子及過氧化氫以微量化學發光法測定。除此之外，我們也將針對茵陳蒿湯的組成中藥，探討它們的抗氧化作用以確定茵陳蒿湯之抗氧化作用的主要來源。我們將測定茵陳蒿、梔子及大黃在試驗管內清除由xanthine/xanthine oxidase誘發的超氧陰離子的作用及其抑制Fecl２所誘發的大白鼠肝細胞脂質過氧化的活性。超氧陰離子將以cytochrome C法測定，而過氧化脂質則以TBA法測定。我們深信此項研究成果將有助於中藥保肝機轉的解明。
關鍵字：茵陳蒿湯,超氧陰離子,過氧化氫,過氧化脂質,抗氧化作用

The study on the antioxidation effect of Inchenhau-Tang

Ming-Fong Chen 
Tainan Municipal Hospital
Recently, free radicals and lipid peroxide are found to be related to the liver damage induced by various chemical agents. Moreover, they are also considered as triggering factors or exerberation factors for viral hepatitis, fatt y liver, ishemia or shock induced liver injury. Therefore, the antioxidants with scavenging effect on free radicals and lipid peroxide are considered to be as useful drugs for liver disease. On the other hand, many Chinese herb drugs used for liver disea se have been found that their pharmacologic effects are due to anti-oxidation activities. In our previous study, we found that Inchenhau-Tang (ICHT) is a most strongest anti-oxidant among twelve herb drug prescriptions which are popularly used in treatm ent of liver disease. ICHT is mostly used in treatment of acute hepatitis with jaundice. Therefore, in this study we will further investigate the scavenging effects of ICHT on the oxygen radicals including superoxide anion (O2－) and hydrogen peroxide (H 2O2) i
關鍵字：Inchenhau-Tang, superoxide anion, hydrogen peroxide, anti-oxidation effect