過敏性鼻炎患者中醫體質類型與基因及蛋白質表現之相關性研究
徐松錕
中央研究院
中醫的診斷利用人體在疾病的表現狀況，將人的體質分成寒、熱等體質類別，利用屬性不同之中藥將不平衡的身體調至平衡以治療疾病。數千年來，體質特性與相關疾病間的關係及其治療方法均已詳細的紀錄在中醫的典籍裡，不同中藥的藥性與其對人體的影響也有詳細記載。為研究中醫的體質類型與疾病表現的關係，我們曾參與兩年的基因體研究計畫，研究中將經由西醫確診為過敏性鼻炎患者，隨機分為二組分別服用寒或熱性中藥方二個月，在服藥前後分別接受中醫體質分型及西醫臨床評估，並用2D-gel做蛋白質分佈的關係研究，以尋找證型、疾病與血清蛋白質的關係。在92至93年的研究中，我們已經分析完成164名患者血清樣本，也發現少數蛋白質（如血色素蛋白）與體質類型相關，但因為2D-gel的解析度相當有限，可能在濾掉白蛋白與球蛋白過程造成微量蛋白漏失，或進行染色分析產生之誤差，因此為了更精確的分析血液中可能與體質寒熱相關之蛋白質含量，我們將採用免疫螢光法（ELISA）來量測血液中與免疫、發炎、細胞間調控有直接相關的細胞激素（cytokines）在不同證型、疾病表現患者的分佈與所扮演的角色。
關鍵字：中醫體質；蛋白質表現；細胞激素；化學激素
The Study of the Relationship between Gene and Protein Expression and the Chinese Medical Constitutional Types in the Patients of Allergic Rhinitis-Role of cytokines
Song-Kun Shyue
Institute of Biomedical Science, Academia Sinica
In Chinese medicine, the patients constitutional types are classified according to their manifestation in disease circumstances, and different types of Chinese herbs are used to treat the patients by correcting the unbalanced status of body. In recent years, instead of the method of studying single gene, many molecular biological information and techniques provide very good methods to analyze the whole expression of the cell. The method to investigate the expression of large quantities of gene and the Chinese medical diagnostic method have similarity that they make use of the characteristics of individual for diagnosis. During year 92 and 93, we have performed a clinical trial and about 164 allergic rhinitis patients were recruited and finished the trial. The patients were grouped randomly into 2 groups, and each took the "cold" and "hot" Chinese medical remedy for 2 months respectively. The constitutional type of each patient was measured by a questionnaire. In order to know the gene related to "cold" and "hot" constitutional types, we used 2D-gel protein analysis to study the proteins variation between different constitutional types, and before and after taking medicines. We have identified three proteins which are strongly related to the "cold" and "hot" remedies taking.
關鍵字：constitutional type；protein expression；cytokine；chemokine
