利用基因晶片系統以及體外細胞培養系統來分析中草藥冬蟲夏草對人類樹突狀細胞免疫功能的影響(2)

許志楧
國立清華大學
中草藥在臨床上的運用已經累積了數千年的經驗。然而，以科學實驗的方法來驗證其對細胞或生物體的影響，仍處於起步階段。近年來，由於醫學科技的進步，中草藥科學化已經是時代的趨勢。為達到此一目標，首先要建立一套以科學實驗的方法，來評鑑中草藥在中醫典籍內所標示的療效。本計畫之目的是要利用基因晶片技術及樹突狀細胞為實驗架構的體外細胞培養系統來分析中草藥冬蟲夏草對人類樹突狀細胞免疫功能的影響。希望藉著觀察冬蟲夏草對樹突狀細胞免疫功能及其基因表現圖譜之影響，進一步有效且可靠的了解冬蟲夏草對增強免疫功能的可能藥理機制。本研究計畫將建立樹突狀細胞的體外細胞培養系統，並利用此一系統來分析中草藥冬蟲夏草對樹突狀細胞免疫功能的影響。在第一年我們如預期順利的建立人類樹突狀細胞體外培養系統、冬蟲夏草的鑑定、冬蟲夏草的品質控管、冬蟲夏草對樹突狀細胞分化與成熟之影響及人類基因晶片的測試實驗。由樹突狀細胞的細胞表面分子得知，冬蟲夏草水萃液會抑制未成熟的樹突狀細胞及成熟過程的樹突狀細胞表面分子的表現。未來將進一步利用其他的免疫實驗確認結果並配合基因表現圖譜的結果找出其相關免疫調控機制。
關鍵字：基因晶片技術；樹突狀細胞；冬蟲夏草

Using Gene Chip Technology and ex vivo Cell Culture System to Analyze the Dendritic Cell Immune Function Affected by Chinese Herbal Medicine-- Cordyceps sinensis(2)

Ian C. Hsu
National Tsing-Hua University, Department of Atomic Science
The Chinese herbal medicine has been administered in traditional Chinese therapy for well over two thousand years. However, the pharmacological study of Chinese herbs on biological objects is still in its infancy. Modernization of Chinese medicine has provoked great interests from bio-medical researchers globally. The objective of this proposal is to analyze the dendritic cell immune function affected by Cordyceps sinensis using cDNA micorarray technology and dendritic cell-based ex vivo cell culture system. The effects of Cordyceps sinensis on cellular immune responses will be measured. Specifically, global gene expression profiles on dendritic cells will be examined with or without the influence of Cordyceps sinensis. These gene expression patterns will be further analyzed in order to study possible pharmacological mechanisms mediated by Cordyceps sinensis. In the first year, we have established a dendritic cell-based ex vivo cell culture system and accomplished phylogenic as well as chemical fingerprinting analysis on Cordyceps sample. The effects of Cordyceps sinensis water extract on the differentiation/maturation process of dendritic cells were being examined. We also performed a mock experiment to test the microarray system.
關鍵字：Gene Chip Technology；Dendritic Cells；Cordyceps sinensis