微陣分析解表藥對巨噬細胞基因活性之影響

張淑芬
臺北醫學大
以中醫角度而言凡以發汗解表使肌表之邪外散或從汗解為主要功能之藥稱為解表藥。解表藥一般適用於外感表證之惡寒、發熱、頭痛、身痛、無汗、透疹、利尿退腫等作用，且根據其性質和臨床作用的不同更分為辛溫解表藥與辛涼解表藥兩類。辛溫解表藥之性味多屬辛溫，其發散作用較強，以發散風寒為主，故又稱為發散風寒藥。辛涼解表藥之性味多屬辛苦寒涼，其發散作用比較緩和，以發散風熱為主，故又稱為發散風熱藥。唯臨床中藥解表用藥物多以病人表狀呈現判斷用藥基準及其療效，缺乏分子層次之學理基礎。由於個體表狀之變化多因細胞內基因表現改變，進而影響功能分子之正常運作，本計劃故擬以基因微陣及轉錄因子微陣分析現常用之中藥解表藥物於細胞中對發炎和抗發炎相關基因如細胞激素及其受體基因和趨化激素其受體基因之表現、以及細胞內轉錄因子是否會因解表藥物之作用而有所改變，自基因層次釐清中藥解表藥物之作用機制。此於分子層次之研究將提供這些解表藥物之可能作用分子機制，以建立中醫傳統理論學說實証基礎。
關鍵字：解表藥、細胞激素基因微陣、趨化激素基因微陣、轉錄因子微陣

Array Analysis of Chinese Diaphoretics on Raw264.7 Cells

Chang, Shwu-Fen
Taipei Medical University
Chinese diaphoretics have been extensively applied for various exterior symptoms caused by pathogenic infection, or other unknown environment factors. Their therapeutic activities are believed to function as sundorifics, pungent and/or bitter Chinese drugs to disperse external pathogenic factors, or bitter drugs to reliever internal heat or cold. Therefore, the diaphoretics has further been classified to drugs capable of reliving exterior symptom caused by wind/cold and by wind/heat. The major objective of this project is to generally profiling the anti-inflammatory, and chemokine factors whose expression was affected in LPS-activated Raw264.7 macrophage cells upon Chinese diaphoretics treatement. Using cytokines/receptors and chemokines/receptors arrays, we will be able to provide the molecular basis for the extensive usage of Chinese diaphoretics on treating exterior symptom-complex.
關鍵字：diaphoretics、cytokine array、chemokine array