中藥減緩老化程序基轉及療效評估(2-2)

楊淑齡
高雄市市立
本研究計畫重點在於篩選抗衰老的中藥，評估其效應，以確立中藥在預防機體老化上扮演的重要角色，並在研究過程中以分子生物學角度，評估探討中藥參與抗衰老過程之可能機轉是否為中藥誘發HSP分子，以減弱自由基，並增加細胞修復能力，而減緩細胞或生物機體的老化過程。
本計畫擬進行二年實驗，以完成藥物篩選及體外培養細胞與動物體內實驗之效應評估，並已於八十九年度完成第一年實驗。
本年度實驗以動物實驗為主，篩選第一年五種方劑中之二種方劑進行動物實驗，給予不同藥物投予劑量，並偵測動物血液及組織中所含HS蛋自及NO量以評估中藥應用於動物體內抗衰老之效應及可能機轉。
關鍵字：老化HSP分子，自由基

The mechanisms and effect of Chinese medicines in attenuation of ageing processes(2-2)

Yang Shu Ling
Kaohsiung Municipal Chinese Medical Hospital
This study was designed to screen the anti-ageing effects of Chinese medicine compounds. The role in resisting ageing was assessed in vitro and in vivo. A point of molecular biological view in studying the mechanism of antiageing was established to confirm if Chinese medicines induce the synthesis of HS proteins which mitigate free radicals and enhance cellular repair and that attenuate the ageing processes.
Whole study was divided two parts. Five types of herbal extract compounds were used to study whether these traditional medicines could provide some effects for antiageing. First year, cultured fibroblasts were treated with herbal extract compounds to access the effect of these compounds on HS proteins synthesis and nitrate existence in vitro. The second year, Wistar rats were treated with herbal extract compounds to access the effect of these compounds on HS proteins, nitrate existence in vivo.
關鍵字：Ageing, HS protein, free radical