以去活化蛋白激酵素及抗氧化酵素系統篩選模式評估龍膽瀉肝湯及當歸龍薈丸之療效
許秀蘊
私立臺北醫
中國傳統藥方龍膽瀉肝湯及當歸龍薈丸均具清熱瀉肝之作用，以治肝膽實火上炎之証。但當歸龍薈丸為大寒大苦之品，非實火壯體，不可輕易嚐試，而龍膽瀉肝湯則作用較為緩和。因此擬從方劑上配方之不同、成分上之差異，就幾種篩選試驗來分辨其差異性，並建立篩選模式。
肝癌為台灣最盛行的惡性腫瘤，而其中以B型肝炎病毒(hepatitis B virus, HBV)感染為眾所皆知的危險因子。利用細胞遺傳學和染色體in situ hybridization 的方法，由染色體變化和B型肝炎病毒基因鑲入的關係探討肝細胞癌化之機序，在細胞分子之層次上HCC 與HBC間有相當的關連性。而將肝癌細胞株HepG2送入B型肝炎病毒基因後, 成功篩選出來的有HepG2-2及HepG2-5二細胞株. 本計畫擬以此HepG2-2、HepG2-5及無B型肝炎病毒基因的HepG2與Hep3B做一對照組，以細胞培養的方式，用中國傳統藥方之龍膽瀉肝湯與當歸龍薈丸(去麝香)，在各種不同極性之萃取物及以luteolin 與quercetin為positive control對照之下，測定細胞毒、DNA fragmentation之分析、總蛋白激酵素之活性、MMPs (matrix metalloproteinase)、黃嘌呤氧化酵素之抑制活性、對超氧化物自由基之驅除作用等，建立一簡易又正確的篩選治療肝炎、肝癌方劑之模式。
關鍵字：龍膽瀉肝湯，當歸龍薈丸，蛋白激酵素，抗氧化酵素
Evaluate the potential effects of the Decoction of Gentianae and Bolus of Angelicae Sinensis and Gentianae by using the screening model on the inactivated protein kinase and anti-oxidative enzyme
Shiow-Yunn Sheu
Taipei Medical College, School of Pharmacy
Decoction of Gentianae and Bolus of Angelicae Sinensis and Gentianae, Chinese traditional herb medicine, both exert the similar effect for Purging Liver-Fire and indicated for sthenic fire in the liver and gallbladder. This Bolus contains potent compounds of bitter and cold character and should not be used by patients unless their physical condition is relatively strong. The action of the Decoction is found to be much milder as compared to the bolus. According to the different composition of prescription, they contain various bio-active components. This project is intended to evaluate the potential effects of those components on hepatoma by using the screening model on the inactivated protein kinase and anti-oxidative enzymes system.
Hepatocellular carcinoma (HCC) is one of the most malignant disease in Taiwan, and hepatitis B virus (HBV) infection is the well-known risk factor. Karyotyping of human liver cancer cell lines and chromosome in situ hybridization of hepatitis B virus (H
關鍵字：Decoction of Gentianae, Bolus of Angelicae Sinensis and Gentianae; Protein tyrosine kinase; Anti-oxidative enzyme system.
