七寶美髯丹的抗氧化作用之臨床研究

陳明豐
台南市立醫
近年來自由基與過氧化脂質被發現與動脈硬化及各種老化現象的進行有密切關係，抗氧化劑被期待開發成為防止動脈硬化及抗老化的藥物。我們在過去的研究中發現，中藥方劑七寶美髯丹在實驗管內具有良好的抗氧化作用，但其在人體內是否具抗氧化作用尚未見任何報導。本研究的目的乃探討中藥方劑七寶美髯丹在人體內的抗氧化作用，我們將以七寶美髯丹及澱粉安慰劑分別投與20名洗腎患者共6個月，並且於藥物投與前及投與後每兩個月一次觀察其臨床症狀的變化並抽血測定其血清過氧化脂質、氧化蛋白質及血清總抗氧化活性。臨床症狀的評估包括全身倦怠感、頭暈、腰酸、下肢無力感及性慾五項目。每個項目將依症狀嚴重度分成四個等級(非常嚴重、嚴重、輕微、無)，並以分數(3,2,1,0)記錄。過氧化脂質乃利用TBA法測定，而氧化蛋白質則以螢光法測定。另一方面，血清總抗氧化活性則以Randox公司之總抗氧化活性(TAS)測定組套偵測。我們將以Student’s t-test對治療組及對照組各項數據的變化進行分析。本研究的結果將提供中藥作為抗氧化劑的有力證據，有助於中藥在抗老化領域的應用。
關鍵字：七寶美髯丹、抗氧化作用、過氧化脂質、氧化蛋白質、血清總抗氧化活性

A clinical study on the anti-oxidative effects of Chi-Bau-Mei-Ran-Dan

Chen Ming-Feng
Tainan Municipal Hospital

Recently free radicals and lipid peroxide had been found to be related to the development of atherosclerosis and elder process. Antioxidants are hoped to be developed as drugs to prevent the development of atherosclerosis and elder process. In a previous study, we found that Chinese herb drug prescription Chi-Bau-Mei-Ran-Dan (CBMRD) has strong anti-oxidative effects in vitro. However, whether CBMRD has anti-oxidative effects in humans is not known. The aim of study is to in vestige the anti-oxidative effects of CBMRD in humans. We will give CBMRD to 20 patients with uremia on long-term hemodialysis for 6 months. And starch placebo will be given to the control group including 20 patients with uremia on long-term hemodialysis. We will observe the change of clinical symptoms of patients and measured the serum concentrations of lipid peroxide oxidative protein and the total anti-oxidative activity. The clinical symptoms to be evaluated include general malaise, dizziness, low back
關鍵字：Chi-Bau-Mei-Ran-Dan, anti-oxidative effect, lipid peroxide, oxidative protein, serum total anti-oxidative activity