大黃主要成份 anthrone 和 anthraquinones 對人類胃癌細胞生長的影響

鍾景光
中國醫藥學
以大黃的主成份anthrone 和 anthraquinones 例如:emodin, aloe-emodin和rhein來測定是否能抑制 人類的胃癌細胞之生長、DNA破壞、細胞週期、染色體及細胞形態的傷害和對乙醯轉移酵素活性的影響，本計畫是利用整個活的人類胃癌細胞經由培養來分析，測定成份emodin, aloe-emodin和rhein的影響則利用兩種主要的儀器，一是流式細胞計數儀(Flow cytometry)，另一是高效液相層析儀(HPLC)。本實驗的目的是決定大黃的主成份 emodin, aloe-emodin和rhein能否抑制人類胃癌細胞的增生及分化
關鍵字：人的胃癌、乙醯轉移酵素、細胞週期

Effects of Da-hung components anthrone and anthraquinones on growth of human stomach cancer cells

Jing-Gung Chung
China medical college
Anthrone and anthraquinones (emodin, aloe-emodin, and rhein), major components of Da-hung, were used to determine inhibition of cell growth, DNA damage, cell cycle, chromosomal damage, and arylamine N-acetyltransferase (NAT) activity in a human stomach adenocarcinoma cell line. The assay system was performed with intact cell suspensions. The assays will be used by flow cytometry and high performance liquid chromatography. The purpose is try to determine whether emodin, aloe-emodin and rhein could inhibit stomach tumor cell proliferation and differentiation.
關鍵字：Human stomach adenocarcinoma, N-acetyltransferase, Cell cycle