台灣本土苧麻屬藥用植物之資源調查及保肝、抗氧化、清除自由基活性評估

顏銘宏
高雄醫學院
本計畫將進行台灣本土原生蕁麻科(Urticaceae)苧麻屬(Boehmeria spp.)藥用植物之市場資源調查及保肝、抗氧化、清除自由基活性研究。緣由起於近四年來台灣南部地區(嘉義、台南、高雄、屏東)民眾盛行以苧麻(Boehmeria nivea var. nivea)根部水抽出物做為保護肝臟、治療肝炎、肝硬化之萬靈飲品，民眾廣為流傳且大量被使用，然真確效果如何眾說紛云，並未獲得證實，恐又將引起社會問題亦未可數。經本研究室的保肝活性研究，證實苧麻根部水抽出物具有抗CCl4、抗Acetaminophen、抗D-galactosamine誘發大白鼠肝毒性的保肝效果，其作用機轉與抗脂質過氧化、清除自由基的功能有關。因此本計畫將調查苧麻屬植物在台灣市場之藥用資源，並計畫進行柄果苧麻(B. blinii var. podocarpa)、密花苧麻(B. densiflora)、台灣苧麻(B. formosana)、苧麻(B. nivea var. nivea)、青苧麻(B. nivea var. tenacissima)的保肝、抗脂質過氧化、清除自由基的活性評估，並記錄劑量依存性(Dose-dependent)之關係，以期確保藥用價值，確定有效安全劑量，開發藥物新資源，維護民眾健康。

市場資源調查將收購台灣市售苧麻屬藥用植物，鑑定基源，找出正條品及「同名異物」及「同物異名」之混淆品。活性研究為：(1)以Cytochrome c還原比色法測試苧麻屬藥用植物對 O2-自由基的清除能力；(2)以FeC12-Ascorbic acid誘發脂質過氧化方法來測試苧麻屬藥用植物的抗脂質過氧化的能力；(3)分離大白鼠肝細胞進行培養，並測試苧麻屬藥用植物對抗Acetaminophen毒殺肝細胞之保護效果，並探討苧麻屬藥用植物能否提升肝內還原型Glutathione之量，降低氧化型Glutathione之量；(4)評估麻屬藥用植物對人類肝癌細胞增生之抑制作用。
關鍵字：苧麻屬、資源調查、組織學研究、保肝、抗氧化、清除自由基

Stueies on Folk Medicine "Boehmeria spp." form Taiwan---The Investigation of Market Resource, and the Biological Evaluation of Hepatoprotective Antioxidant and Free Radical Scavenger

Yen Ming-Hong
School of Pharmacy, Kaohsiung Medical College
In the present program, the folk-medicine "Boehmeria spp." (Urticaceae) from Taiwan will be investigated to the market resources and their biological activity of antioxidant and free radical scavenger. The Boehmeria spp. included : B. blinii var. podocarpa, B. densiflora, B. formosana, B. nivea var. nivea, and B. nivea var. tenacissima. 

The market resources investigation will be examined with pharmacognostical and anatomical studies to find the correct origin. Furthermore, to evaluate the pharmacological effects of Boehmeria spp., the following experimental models were employed : (1)anti-lipid peroxidative effect induced by FeCl2-Ascorbic acid model; (2) the active oxygen scavenging activity by using cytochrome c reduced colorymetry model; (3) investigated primary liver cells cultured with acetaminophen and crude drug extracts, and study the effect of reduced-, oxidized-glutathione level in rats liver; (4) the antiproliverative effect of hepatocarcinoma cell were also investiga
關鍵字：Boehmeria spp.,Resource investigation,Anatomy, Hepatoprotection, Antioxidation,Free Radical scavenger