腎氣丸對塵璊激發氣喘天竺鼠呼吸道發炎與免疫之影響

高尚德 
中國醫藥學
中醫古典醫籍中，對於氣喘之病機、症狀早已有詳細記載，並累積了豐富的臨床經驗，近年來有關氣喘中醫治療文獻，大部份著重於臨床療效評估，利用氣喘動物模式，了解氣喘治療方劑對氣喘動物呼吸道阻力、呼吸道發炎細胞與呼吸道反應性之影響，進而探討這? `用治療氣喘方劑之治病機轉，則較少發表。
本研究擬利用塵璊（mites）純化液誘發天竺鼠形成氣喘動物模式，藉身體體積量法、肺泡沖洗液分析、分子生物學、肺部病理組織檢查等方法，研究氣喘天竺鼠，在氣喘緩解期以中醫常用於氣喘緩解期調整過敏體質之補腎方劑-腎氣丸調養後其呼吸道阻力與呼吸道? o炎細胞之改變，以了解腎氣丸對氣喘動物呼吸道阻力、呼吸道發炎細胞、發炎介質（PGE2、LTC4）呼吸道反應性、CD4+T cell、支氣管上皮細胞凋亡（apoptosis）與肺部病理組織之影響，期望能了解腎氣丸治療氣喘之機轉。
關鍵字：塵璊、呼吸道阻力、氣管肺泡沖洗液分析

The effect of Shen-qi-Wan on inflammation of respiratory tract and immnology after Mites Challenge of asthmatic Guinea Pigs

Shung-Te Kao 
China Medical College
There were many detail records about the mechanism、clinical symptoms and treatment of bronchial asthma in traditional Chinese medical books. Recent years, the most papers of Chinese medical treatment in bronchial asthma emphasized largely in clinical evaluation. There were few reports utilizing asthma animal model to study the mechanism of Chinese medical formula in bronchial asthma through understanding Chinese medical formula affect the change of respiratory resistance and inf lammatory cell of respiratory tract of asthmatic animal. In this research, we use mite to induce Ginea Pig to establish asthmatic animal model, and we evaluate the change of respiratory resistance and inflammatory cell of respiratory tract of sensitiz ed Ginea Pig before and after Shen-qi-Wan treatment by bodyplethysmography、bronchoalveolar lavage Molecular biologic method and bronchial pathology to understand the effect of Shen-qi-Wan on respiratory resistance respiratory tract inflammation bronchia l reacti
關鍵字：Mites、Respiratory resistance、Bronchoalveolar lavage