由中藥草中分離能提昇輻射治癌效果的輻射增敏劑3-2）

譚世特 
清華大學
本計畫之全程目的在：篩選中藥草以分離出天然的、能增加癌細胞輻射敏感度的藥劑（簡稱輻敏劑）。
計畫之短程目的在：建立以微生物細胞為工具、快速且有效之篩檢系統，配和癌細胞株之運用，進行大規模篩檢輻敏成分的工作。本計畫由三個不同實驗室合作，結合藥物化學，應用微生物學、與輻射生物學的知識。
計畫第一年將建立良好輻敏效應的測試及輻敏劑篩選細胞系統，並且完成五十種中藥純化樣品及數種粗萃出物的測試。細胞系統包括抗輻射奇異球菌、大腸桿菌及酵母菌等微生物與哺乳類細胞株。
計畫第一年的前四個月中，我們已完成五十多種樣品的製備，並且也完成抗輻射奇異球菌及人類直腸癌細胞株之輻射敏感度的測試（採用已知的輻敏藥劑為正控制組）。在第一年餘下的時間，我們將測試五十種樣品對細胞之毒性及輻敏效果。
計畫第二年中，我們將製備及測試更多的中藥草樣品，有輻射效果者，將進一步測試他們對不同癌細胞株（如肺癌、乳癌等）之輻敏效果。有效的篩選系統將進一步改良並予以發表。
關鍵字：癌症治療、中藥輻射增敏劑、抗癌中藥

Screening the Chinese medicine for radiation sensitizers in radiotherapy for human cancers

Shieh Te Tan 
National Tsing Hua university
The long-term goal of this project is to isolate,from Chinese medicine,the natural compounds that can be used as radiosensitizer in the treatment of human cancers by radiotherapy.
The immediate aim is to establish a rapid and effective screening protocol for such compounds using microbial systems and a sensitive confirmatory mammalian system using human tumor cell lines.The initial microbial system coupled t o the mammalian system will enable us to screen a large number of samples.This project combined the effort of three laboratories with different but interactive expertise in medicinal chemistry of natural compounds,applied microbiology,and molecular radia tion biology.This well-assembled collaboration will greatly enhance the progress and success of the project.
In the first year,the cultural conditions and the measurement of radiation sensitivity of different cell types will be established. The cell types used will include Deinococcus radiodurans,Escherichia coli, and Saccharomyc
關鍵字：Cancer therapy,Chinese medicine as radiosensitizers,Anticancer Chinese medicine