由中藥草中分離能提升輻射治癌效果的輻射增敏劑

譚世特 
國立清華大
本計畫之全程目的在：由中藥草中分離出天然性的癌細胞輻射增敏（簡稱輻敏）藥劑。短程目的在：建立以微生物細胞為工具、快速且有效之篩檢系統，配合癌細胞株之運用，進行大規模的篩檢工作。本計畫由三個不同實驗室合作，結合藥物化學、應用微生物學、及哺乳細胞輻射? 耵姥漯壅恁C

計畫之實施方法：首先製備中藥草萃出物樣品，其次利用操作簡便之單細胞微生物，包括大腸桿菌、抗輻射奇異球菌、及酵母菌建立快速篩選系統。若某一藥草萃出物樣品在這些微生物細胞系統中任何一種顯現有輻敏效果，則將直接以癌（肺癌）細胞株進行輻敏效果測試。對已經純化的中藥成分亦可直接運用於肺癌細胞株測試。第一年主要工作項目包括：(一)樣品製備、(二)細胞輻射照射條件設定、(三)中藥樣品的細胞毒性測估、及(四)中藥樣品之細胞輻敏效果之測定。

全程計畫共三年。第一年完成至少五十種以上的中藥純化樣品及數種粗萃出物樣品之測試，並建立良好的篩選系統。中後期的工作除繼續擴大樣品數目外，將視實際進展進行(一)初步測試之有效藥物對各類不同的癌細胞是否有同樣功效；(二)輻敏劑化學構造修飾以增強藥效並降低毒性；(三)動物實驗。

關鍵字：中醫、輻射增敏劑、篩檢方法

Screening the Chinese medicine for radiation sensitizers

This three-year project was designed to screen Chinese herbal extracts for radiation sensitizers useful in radiotherapy for human cancers. This project will be a joint effort of three laboratories with expertise in pharmaceutical chemistry, applied microbiology, and mammalian cell radiology. In the first year, work will be focused in establishing rapid and economical cell-based screening procedures.

Three different microorganisms including Escherichia coli, Deinococcus radiodurans, and Saccharomyces cerevisiae, which cover a broad spectrum of cellular mechanisms for radioresistance will be used as the test organisms in screening crude herbal extracts (or purified natural compounds if available). Purified compoundes or crude extract samples showing positive results in microbial system(s) will be further tested in a mammalian cell system.

Experiments to be done in the first year include (i)Preparation of samples, (ii)optimization of conditions for radiation and drug treatme
關鍵字：Chinese medicine; radiation sensitizer; screeing method