建立抗癌中藥篩選平台：以肺腺癌為例

近年來世界各國肺癌的發病率和死亡率急劇上升，台灣地區的研究顯示罹患肺癌之危險度較他國偏高，尤以女性及年輕男性患者增加最為顯著。由於肺癌的預後差，初期臨床症狀不明顯，通常診斷發現時已屬中晚期。在肺癌的種類中，其中肺腺癌是目前肺癌的主要類型，使用傳統毒殺性化學治療藥物效果不佳，同時副作用又很大，而新研發上市之標靶藥物，陸續進行試驗之結果仍不令人滿意，除了治療費用驚人、療效不如預期外，也仍有不少副作用產生。本計畫的主要目的為建立一個有效率的平台，結合中醫臨床的診治經驗，篩檢出可以有效對抗肺腺癌的中藥。本計畫將建立有效率的平台，篩檢對肺腺癌細胞有顯著毒性的中藥，以找出可能有效抑制肺腺癌的中藥。所篩選出來的藥物，在未來將研究這些中藥對肺腺癌的抑制生長或引發細胞凋亡的相關蛋白的表現及分子機轉，以及對在細胞癌化及轉移中特有蛋白的表現調控。此計畫預計完成以下目標：運用先前我們已經建立一套用來篩選治療肝癌中藥的平台，將修改該平台，以篩選可能用來治療肺腺癌的中藥。主要目標將放在：一、 有效抑制細胞生長，及引發細胞凋亡的中藥。二、 抑制細胞爬行（metastasis轉移）能力的中藥三、 抑制與轉移有相關蛋白表現基因，如FAK，MMPs等的中藥未來研究方向：經篩選出有專一療效的可能中藥後，進一步探討個別中藥對肺腺癌細胞株中與癌化有關的基因調控機轉，以確定中藥的有效性與作用機轉，對有效的藥物，更進一步的規劃動物或臨床實驗。 相對於現在臨床上使用於治療肺癌的單標靶藥物，中藥具有多標靶的治療功能，配合中醫的理論，可以將該中藥的藥性發揮，並減低副作用。我們期望從這些中藥中找出能有效的抑制肺腺癌細胞的生長、轉移，並能有效調控與肺腺癌在動物體內的成長與相關的基因表達的中藥。
關鍵字：

Screen effective herbs used in Chinese Medicine for lung adenocarcinoma therapy

The incidence of lung cancer has been rising very fast worldwide. In Taiwan, the incidence is much higher than other country, especially in women population. Due to poor detection tools, most of the patients were detected as lung cancer at very late stage and with very poor prognosis.Amoung lung cancer, adenocarcinoma is the most popular cancer. Most chemotherapy drugs do not have significant treatment efficacy while with seveal side effects. Recently, the target drug was developed with very high price and limited efficacy accompanied with some side effects reported. This project is to establish a plateform to screen herbs used in Chinese medicine which have efficacy on lung adenocarcinoma therapy. We want to establish the screening plateform to scrutinize the efficacy of identified herbs, which were suggested to have efficacy on lung adenocarcinoma therapy and were pre-screened by Chinese medicine clinical results.
關鍵字：