龍膽瀉肝湯對紅斑性狼瘡小鼠之調控機制研究

李宗諺
長庚大學
全身性紅斑性狼瘡是一種慢性、多器官侵蝕、系統的發炎性，它和免疫系統失調及廣泛性地免疫複合體沈積有關，同時可以見到細胞激素和發炎細胞共同產生的炎症反應。其病理機轉最主要是導因於身體所產生不同種類的自體抗體，以致於造成身體各個系統及器官的功能喪失會危及生命的疾病。
龍膽草(Gentianae Radix)是中醫臨床最常用的清熱瀉火劑之一，原為瀉肝膽實火、清下焦溼熱而設，但目前所使用的範圍則越來越廣，既廣被報導具有抑制脂質過氧化的作用，又兼具免疫調節的功能，但真正在實驗性紅斑性狼瘡的作用機制卻一直缺乏科學性的探討。
本計劃以MRL lpr/lpr小鼠為自發性的自體免疫疾病動物模式，小鼠在長大的過程中會出現類似人類全身性紅斑性狼瘡的症狀。實驗動物與以餵食龍膽草及其純化物(gentiopicroside)八週後，收集尿液、血液與腎臟組織進行病理檢驗與免疫分析，包括抗雙股去氧核糖核酸抗體、測量淋巴結及尿蛋白、腎臟組織切片等。收集血清進行一氧化氮與脂質過氧化分析。其次在本研究中，我們嘗試以蛋白質體學的方法來探討對紅斑性狼瘡動物模式有療效的指標蛋白。
藉由此研究，我們希望能進一步評估龍膽瀉肝湯具有治療人類全身性紅斑性狼瘡的潛力。
關鍵字：紅斑性狼瘡、龍膽瀉肝湯、自體免疫疾病

Modulated effects of Longdan Xiegan Tang on systemic lupus erythematosus of MRL lpr/lpr mice

Tzung-Yan Lee
Chang Gung University
Systemic lupus erythematosus (SLE) is a chronic multisystem disorder of presumed autoimmune origin in which cytotoxic antibodies, or circulating immune complexes, give rise to tissue damage often resulting in end organ disease and even mortality. 
Gentianae Radix is a herbal medicine with purge gandanshihou (瀉肝膽實火) and clear xiajiaoshire (清下焦濕熱) effects. In addition, which has significant effect on oxygen free radicals and lipid peroxidation. In the present study we investigate its immunomodulatory effect on inhibiting autoimmune disease in MRL lpr/lpr mice. MRL lpr/lpr mice treats daily with water extract of Gentianae Radix or gentiopicroside for 8 weeks. We will study the role of immunological effect of Treg and pathological effect of kidney tissue and progression in anti-ds-DNA production, proteinuria, lymphadenopathy and herbal medicine administration. The protein expression profiles in kidney tissue that are associated with the physiological and pathological change during SLE progression will be analyzed by two-dimension gel electrophoresis and image analysis. Furthermore, we will assess the possible mechanism underlying the therapeutic effect of Gentianae Radix in blood nitric oxide and lipid peroxidation levels. The study would fill a long-existing gap in Chinese medicine in the pathophysiological disorder of SLE. It may also lead to the discovery of potentially useful herb drugs for treating SLE in human patients.
關鍵字：Systemic lupus erythematosus、Longdan Xiegan Tang、autoimmune