按 Enter 到主內容區
  • facebook
  • line
  • twitter
  • 列印
  • 回上一頁

CCMP99-RD-056 利用代謝體學建立龍膽中藥材生產品質管制種植規範(GAP)

  • 資料來源:中醫藥司
  • 建檔日期:102-08-12
  • 更新時間:106-06-15

利用代謝體學建立龍膽中藥材生產品質管制種植規範(GAP)

張文德
中國醫藥大學
GAP(Good Agriculture Practice)為國際要求的優良種植規範,主要分三個體系:藥材質量體系、操作規範及後期加工標準。從育種土壤開始,到施肥、農藥、栽培、採集等各個環節都需要有標準規範。中藥材龍膽原植物為粗糙龍膽(Gentiana scabra Bunge),具有保肝利膽、清熱瀉火之功效。現代科學化的藥理研究亦證明具有保肝、利膽、健胃、抗發炎、抗過敏及骨骼肌鬆弛等作用。台糖公司研究所已成功地利用組織培養技術自龍膽出瓶苗株中選拔耐熱個體株,於控制環境下建立龍膽組織培養母瓶並進行增殖,降低產程中微生物污染之損耗,提高組織培養苗生產效率。並在溫室種植試驗,對栽培期間之病蟲害及環境因素進行病原性測定及防治管理,提供龍膽產業化生產收穫規範之依據。
本計畫與台糖公司研究所合作,利用台糖公司的離蔗農場繼續進行龍膽草大量的田間栽培,依照中藥材GAP規範的條件要求,建立台灣本地之龍膽中藥材生產品質管制種植規範(GAP)。另外,本計畫將從中醫藥專業角度,配合現代之代謝體學之新觀念,針對台糖公司研究所所生產之龍膽藥材,與中國生產之龍膽藥材,以NMR、UPLC進行植物代謝體學之分析研究,探討兩者間的差異度。同時進行相關的藥理活性試驗,如:大鼠之胃腸蠕動試驗、急性肝炎試驗以及抗發炎實驗等,以系統性生物學的觀點,闡明龍膽藥材成分與活性的關聯性,探討台灣生產之龍膽藥材取代中國進口龍膽藥材的可能性,並建立高品質龍膽藥材之供應體系,為台灣精緻農業開創另一新事業。
關鍵字:龍膽、代謝體學、GAP、中藥材生產品質管制種植規範

Estabolishment of GAP on Gentiana scabra Using Metabolomic Strategy

Wen-Te Chang
China Medical University
There are three main aspects involved into the international guideline of Good Agriculture Practice (GAP), including materials quality, manipulation guides and processing standard. Each step has to be set up the standard guideline from breeding, propagation, cultivation management and diseases control. Gentiana scabra is the original plant for Chinese medicinal Long Dan (Radix Gentianae) which acts on the liver and gallbladder channels. It has the functions of quenching fire of liver and gallbladder and eliminating damp-heat. The effects in pharmacology are stomachic, hepatoprotective and choleretic, hypotensive and diuretic, as well as antibacterial and anti-inflammatory. Taiwan Sugar Research Institute has successfully screened the heat-resistant plant from mericlones of G. scabra using tissue cultures and propagated under controlled systems to enhance the efficiency of mericlones production. Then, the detection and prevention arrangements were conducted for blight and environmental factors during the cultivation in the greenhouse. These findings are able to be a basis for production and harvest of G. scabra in industrial scale.
This project will be cooperated and carried out with Taiwan Sugar Research Institute. The main aim of this project is to establish the GAP of G. scabra for large scale field production using the cane farms in Taiwan following the GAP guideline of Chinese medicinal plants. In addition, based on the point of view of Chinese medicine and the new concept of modern metabolomics, the materials of G. scabra produced by Taiwan Sugar Research Institute will be compared with the materials from China using the analysis of plant metabolomics by NMR and UPLC. The pharmacological activities of G. scabra, such as stomach empting test, acute hepatitis test and antiinflammation test, will be conducted simultaneously. It will be able to explain the relationship between components and bioactivities of G. scabra under systems biology. The final goal of this study is to build up the supply system of high quality G. scabra material to instead of the imported material from China and create a new business of exquisite agriculture in Taiwan.
關鍵字:Gentiana scabra, Metabolomics, Good Agriculture Practice