建立中藥抗流感病毒之篩選平臺─藥效及作用機制之評估

陳志豪
中山醫學大學
流感為台灣重要的呼吸道疾病，除了引起嚴重的呼吸道症狀外，甚至會造成全世界大流行及死亡。雖然現在已經發展出有效的抗流感的藥物，如克流感及瑞樂沙等，但其缺點價格昂貴、並長期需要依賴國外供應，更重要的是不斷有抗藥性的流感病毒產生。因此，有必要發展新的抗流感的藥物。在我國，中藥一直是西醫外的另一選擇，以往也有很多評估某些中藥抗病毒之研究，但都屬於比較幾種中藥的小規模研究而已。本計畫則是與中國醫藥大學中國醫學研究所合作，並由該所提供約329種中藥中，期能找出能有效抗流感病毒的藥物。本計畫共分二年進行：第一年，針對A/H1N1、A/H3N2及B型流感病毒，在本實驗室現有之約329種中藥萃取物中，以血球凝集抑制試驗、微量中和試驗等，初步篩選具有抗流感病毒活性的藥物，預估可篩選出10~40種比較有潛力的中藥。再從這些中藥中定出IC50，並利用感染雞胚胎蛋模式之實驗，評估中藥在雞胚胎蛋中抗流感病毒之效力如何。第二年則將一年比較有效的中藥，作單味藥活性物質的分析，進行成分的拆解 (包括solvent partition、TLC等), 以其化合物進行上述測試。
關鍵字：流感、抗病毒、雞胚胎蛋、活性成份

Establishment of screening platform of Chinese herbal medicine for anti-influenza: evaluation of the effect and mechanism

Chi-Ho, Chan
Influenza is one of important respiratory diseases in Taiwan. Besides serious respiratory symptoms, influenza virus can cause pandemic and death. Although anti-influenza drugs like Tamiflu and Relenza, have been developed successfully, the costs of these drugs were expensive. Other disadvantages include the dependence of exportation from foreign countries and the emerging of drug resistant strains. For these reasons, it is crucial to develop new anti-influenza drugs and the Chinese herb medicine is an alternative choice in Taiwan. In previous studies, several herbs have been found to have anti-influenza activities, but it only belonged to a small scale study. In this program, we cooperate with the research team of Graduate Institute of Chinese Medical Science, China Medical University and will screen anti-influenza Chinese herb medicine from approximately 329 species of Chinese herb medicines. For the first year, screening drugs from 329 species of Chinese herb medicines against influenza viruses, A/H1N1, A/ H3N2 and B, by using hemagglutination-inhibition test and microneutralization test. In addition, IC50 and the anti-viral effect in chick embryonated eggs will be estimated. For the second year, we will try to separate and analyse the active compound from the candidate drugs.
關鍵字：Influenza, antivirus, chick embryonated egg, active component