減肥中草藥之藥效與肝腎毒性的評估(2-1)

劉怡旻
大仁科技大學 藥學系
本計畫擬利用兩年的時間，針對市面上常用於減肥的中草藥藥材炮製品，評估是否具有預防高熱量飼料引至實驗動物罹患肥胖症之效果，同時針對長期服用這些待測中藥及其炮製品後是否可能引至肝毒性、腎毒性進行評估。第一年度計畫將針對具有改善高血脂作用的何首烏與柴胡以及藥材炮製品，製首烏與醋柴胡進行評估；第二年度計畫則針對具有利尿作用的澤瀉和決明子，與其藥材炮製品，分別為鹽澤瀉與炒決明子進行探討。本計畫將藉由每星期檢測高熱量飼料引至肥胖大鼠血液中肝功能生化指數glutamic pyruvic transaminase (GPT)及glutamic oxaloacetic transminase (GOT) 的變化，配合肝臟膠原蛋白含量之定量分析所得結果，評估待測中藥材及其炮製品對實驗動物肝臟功能的影響。另外，藉由血漿肌酐酸(serum creatinine)、血尿素氮(blood urea nitrogen)濃度的變化以及腎臟的組織病理切片，評估實驗動物腎細胞腫脹及傷害的程度。同時，探討腎臟中具腎功能調節作用的因子如轉化生長因子(TGF-β1)或是膠原蛋白(collagen IV)蛋白量的表現，以解明待測中藥材及其炮製品引致肝腎毒性之可能病理機轉。本項計畫的完成將有助於證實這些待測藥材與其炮製品是否具有預防高熱量飼料引至實驗動物罹患肥胖症之效果，並找出可能產生療效的劑量。此外可瞭解長期服用這些待測中藥及其炮製品後是否可能引至肝毒性與腎毒性。所得結果將可提供產官學各界有關中藥服用安全評估、政策的建立及研究方向的參考。
關鍵字：減肥；何首烏；柴胡；澤瀉；決明子；肝毒性；腎毒性

Evaluation on the anti-obesity efficacy as well as kidney and liver toxicity induced by Chinese medicinal plants (2-1)

I-Min Liu
Department of Pharmacy, Tajen University
The research is the two-year plane. The aim of the research is going to evaluate the effects of herbal medicines and the processed products that commonly used in weight reduction, on prevention of high energy diet-induced obesity in rats. In addition, the possible hepatotoxicity or nephrotoxicity induced by long-term administration of these anti-obesity herbal medicines and its processed products will be evaluated. In the first year, the anti-obesity efficacy and the toxicity associated with herbal with hypolipidemic activity, such as Polygoni Multiflori Radix and its black bean sauce-processed product, as well as Bupleuri Radix and the vinegar-processed product, will be evaluated. In the second year, the anti-obesity efficacy and the toxicity related with herbal diuretic, including Alismatis Rhizoma and salt-processed product, or Cassia Seed and firing-processed products, will be investigated. During the experimental period, the plasma levels of glutamic pyruvic transaminase and glutamic oxaloacetic transminase will be measured, and hydroxyproline content will be determined in liver tissues to evaluate whether the hepatotoxicity will be induced by the tested herbal medicines and the proceed products. Kidney damage induced by the tested herbal medicines and the processed products will be determined by chaning in the levels of blood urea nitrogen and plasma creatinine concentration. The biopsy will be further performed to evaluate the pathology of hepatotoxicity and nephrotoxicity. Furthermore, the influences of the tested herbal medicine on the levels of transforming growth factor-β1, a protein related with extracellular matrix synthesis, as well as the changes of collagen IV, will be determined by immunohistochemistry staining. The results will provide valuable information about safety of tranditional Chinese medicine for both academic and industrial parties and maybe helpful for the establishment of related policy.
關鍵字：weight reduction, Polygoni Multiflori Radix, Bupleuri Radix, Alismatis Rhizoma, Cassia Seed, hepatotoxicity, nephrotoxicity