台灣本土中草藥之應用與開發－臺灣鉤藤
李美賢1 侯文琪1 林乾閔2
1臺北醫學大學生藥學研究所 2署立雙和醫院神經外科
中藥材鉤藤為為茜草科 (Rubiaceae) 植物，基源主要是中國大陸的Uncaria rhynchophylla（Miq.）Jacks.，屬於平肝熄風藥。藥效為清熱平肝，熄風定驚，主要用在清熱祛風，外感風熱，小兒驚癇，血壓偏高，頭昏目眩。本計畫以台灣本土中草藥之應用與開發為主要目標，為達成此目標，我們選擇農委會種苗改良繁殖場提供之田間種植的台灣本土中草藥—臺灣鉤藤（Uncaria hirsute Haviland）為材料，進行其活性成分抽取及神經保護之探討及應用。並比較鉤藤與臺灣鉤藤於活性成分及神經保護作用之異同，達到應用台灣本土中草藥之目標。本計劃擬完成的項目分列如下：1. 鉤藤 (U. rhynchophylla) 藥材與台灣本土中草藥－臺灣鉤藤 (U. hirsuta) 之基原鑑定與不同溶劑萃取物之製備。2.以進行延緩老化之神經保護動物模式試驗為平台，進行活性萃取物之評估。3.利用液-液部分劃分、管柱層析等方法進行活性萃取物之部分純化及其指標成分化學指紋圖譜分析。4.最具活性之目標物，進行化學指紋圖譜分析。5.最具活性之目標物，進行人類神經母細胞瘤細胞 (SH-SY5Y) 體外試驗機轉探討。
關鍵字：台灣本土中草藥；臺灣鉤藤；神經保護
Development and utilization of indigenous Taiwanese herbal medicine –Uncaria hirsuta Haviland
李美賢1 Mei-Hsien Lee 1 Wen-Chi Hou 1 Chien-Min Lin 2
1 Graduate Institute of Pharmacognosy,Taipei Medical University 2Department of Neurosurgery, Taipei Medical University - Shuang Ho Hospital
Chinese herbal medicine - Uncaria Ramulus et Uncus is a Rubiaceae plant. The sources in mainland China is mainly Uncaria rhynchophylla (Miq.) Jacks. In traditional utility, it belongs to anticonvulsives or drugs for subduling the hyperactivity of the liver and the endogenous wind. Efficacy for clearing up heat, subduing hyperactivity of the liver, and relieving convulsion. It mainly used in heat dispelling, exogenous wind-heat, shock in children epilepsy, high blood pressure, dizziness. In this project, the application of Taiwanese Chinese herbal medicine development as the main objective, to achieve this goal, we chose Uncaria hirsuta Haviland from Taiwan Seed Improvement and Propagation Station, COA as the material. The active ingredient extraction, neural protection, and its application will be evaluation in this project. And to compare with the active ingredients and neuroprotective effects of the two materials from different sources. Upon completion, we hope to achieve the goal of application of Taiwanese herbs. The program intends to complete the project are as follows: 1. The original identification and preparation of extracts in different solvents from U. rhynchophylla and U. hirsute; 2. The use of in vivo animal model as a platform for activity assessment of the extracts; 3. To obtain the active partially purified extracts or pure compounds by using liquid - liquid partition, column chromatography, or other methods. The chemical fingerprint analysis of index components of the active materials will be analyzed; 4. To assess the neuroprotective effect in in vitro and their chemical fingerprint of the most active target; 5. To investigate the mechanism of the most active target in human neuroblastoma cells (SH-SY5Y) in vitro.
關鍵字：Taiwanese herbal medicine; Uncaria hirsute; neuroprotection
