表面電漿波相位生物感知器對中藥之單一成份抑制貝它類澱粉蛋白聚合化效用之量測

周晟
國立陽明大
目前老年性疾病中的阿滋海默氏症患者人數逐漸增加，患病數年後因喪失對身體控制能力而失去生命。阿滋海默氏症患者腦部散佈著類澱粉沉積(amyloid deposits)的病理特徵。類澱粉沉積的主要成分為Aβ (β-amyloid protein)，可溶的(soluble)Aβ並不具有毒性，必須在Aβ聚合形成纖維束(fibril)後才有神經元毒性。
Aβ纖維束是形成阿滋海默氏症主要致病原因的來源，因此抑制纖維束的形成，成為治療阿滋海默氏症的一個重要途徑。過去對Aβ纖維束形成動力學的研究，均無法提供Aβ形成纖維束前的中間產物(intermediate)，在早期纖維束形成前的動力學描述。在此我們將利用表面電漿波生物感測器，利用來自生物體本身具備的高特異性，高靈敏度，來接受待測物的選擇而產生專一性的反應，同時透過快速、即時觀察、不需標示(labeling)的優點，我們可以即時觀察並記錄生物分子間的相互作用情形而進行動力學之分析，本研究將對可抑制Aβ1-42體外聚合反應的中草藥粗萃物，提供對早期Aβ纖維束形成動力學的研究，將有助於開發新的治療AD之藥物。
關鍵字：生物感知器,貝它類澱粉蛋白,表面電漿波

Phase-sensitive surface plasmon wave biosensor for identification of Chinese herbs with inhibitory activity on aggregation of β-amyloid

Chien Chou
National Yang-Ming university
Effectiveness of single constituent of Chinese herbs with inhibitory activity on ?-amyloid polymerization by use of phase-sensitive surface plasmon wave biosensor
Alzheimer’s disease (AD) is one of the leading causes of death in the elderly of the developed world. This widespread progressive neurodegeneration is characterized by the presence of proteinaceous deposits in the brain described as amyloid on the basis of tinctorial properties. Substantial evidence indicates that aggregation of β-amyloid (Aβ) peptides (Aβ1-40 and Aβ1-42) resulting neuronal toxicity probably play a causative role in the etiology of AD.
Past studies of the kinetics of Aβpolymerization, utilizing turbidity, sedimentation and dye binding, could only provide information on the appearance of high molecular weight aggregates. Rate constants could not be determined by these approaches. In contrast, surface plasmon resonance(SPR) biosensor can be used to monitor binding events in real time without labelin
關鍵字：biosensor,beta-amyloid,surface plasma wave