中藥材「防風」之量產繁殖

張宏祺
朝陽科技大學
防風（Saposhnikoviae Radix），據《中國藥典》收載為繖形科（Umbelliferae）防風（Saposhnikovia divaricata (Turcz.) Schischk））之未抽花莖植株的乾燥根，產於中國大陸東北和華北等地，別名：關防風、東防風等。由於原生地無度亂挖濫採，野生資源遭受嚴重損壞，更影響生態環境，使產量遠不能滿足藥業發展對這一大宗中藥的需求。本研究運用植物組織培養技術建立防風再生系統，並以生物反應器進行量化生產之可行性評估，再以HPLC分析如4’-O-β-glucosyl- -5-O-methylvisamminol、cimifugin 、prim-O-glucosylcimifugin等主要活性物質之含量分析供建立品質控制之依據。
關鍵字：防風、大量繁殖、生物反應器、品質控制

Masspropagation of Saposhnikovia divaricata Schischkin

Hung-Chi Chang
Chaoyang University of Technology
According to the Chinese Pharmacopoeia, Saposhnikoviae Radix (Fang feng) is the dried roots of Saposhnikovia divarica (Turcz.) Schischk, which is a perennial herb belonging to the family Umbelliferae. The medicinal material is collected in autumn before bolting and produced in Northeast and North of mainland China. Common names: Guanfangfeng, Dongfangfeng. However, the natural sources of S. divaricata have been seriously damaged because of environmental destruction and the destructive collection of the roots in recent decades. Production can not meet the pharmaceutical development of demand for this staple of Chinese medicine. The purpose of this study is to develop a regeneration system for S. divaricata, the efficient commercial micropropagation system by bioreactor, and HPLC analysis of the active substance content, such as 4’-O-β-glucosyl-5-O-methylvisamminol, cimifugin, prim-O-glucosylcimifugin, for quality control, for quality control.
關鍵字：Saposhnikovia divaricata; micropropagation; bioreactor; quality control.